WebbType I restriction enzymes possess both restriction and modification activities. In this case, the restriction will depend upon the methylation status of the target DNA sequence. … WebbA 1.4-kb PstI-Hind111 fragment was ultimately identified which contains an open readingfram=ding for a polypeptide of 146 amino acid residues that was highly homologous to the D. vulgaris flavodoxin, sharing a sequence identity of 55%.
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Webb5 maj 1993 · The patterns of sequence similarity in pairwise comparisons of the family members suggest that gene conversion may have played a role in the evolution of this GST multigene family. We propose that the Drosophila gst D genes provide a unique system for studying GST gene regulation, in vivo physiological functions, and evolution of … WebbThe SacI site can be used as the 3' cloning site, however, a KOZAK sequence or Shine-Dalgarno sequence should be placed at the 5' end of your gene, depending your … tata cara mandi wajib laki-laki yang benar dan sah
52: DNA Restriction and Electrophoresis - Biology LibreTexts
Webb9 feb. 2024 · Gene of interest (214bp) amplified with Hind111 site in forward primer and reverse primer. Infectious clone linearised with Hind111 digestion (Digestion confirmed by running uncut infectious... WebbQuestion: Item 7 Part A n a control experiment, a plasmid containing a Hindlll recognition sequence within a kanamycin resistance gene is cut with Hind religated, and used to … WebbIn a control experiment, a plasmid containing a HindIII recognition sequence within a kanamycin resistance gene is cut with HindIII, re-ligated, and used to transform E. coli K12 cells. Kanamycin-resistant colonies are selected, and plasmid DNA from these colonies is subjected to electrophoresis. 16部委 数据安全