Myh11-creert2 rosa
Web019079 B6.FVB-Tg(Myh11-icre/ERT2)1Soff/J SMMHC-CreER T2 transgenic mice allow sufficient Myh11 -driven tamoxifen-inducible Cre expression in smooth muscle cells (SMCs). In 2024, expanded quality control screening at The Jackson Laboratory determined the … Web31 mrt. 2024 · DP1 f/f Myh11 CREERT2 mice were crossed with Gt (ROSA) 26Sortm9CAG-tdTomatoHze (R26 mTmG/+) mice (Jackson Laboratory, no. 007909) to generate DP1 f/f Myh11 CREERT2 R26 mTmG/+ mice for VSMC lineage tracing.
Myh11-creert2 rosa
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Web3 feb. 2024 · TgMyh11 CreERT2; Tcf21 flox/flox;Rosa26Sor tm14(CAGtdTomato)Hze; Apoe −/−: Male (Myh11 CreERt2 transgene is linked to chromosome Y: HFD (0.15% chol, 21% milk fat, Dyets TD 31359001); SD: 8–16 w: scRNA-seq; CITE-seq: Enzymatic digestion … WebStaining of mouse bone marrow cells with Anti-Mouse CD34 FITC (Product # 11-0341-82) and Mouse Hematopoietic Lineage Biotin Panel (Product # 88-7774-75) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis. …
Web8 okt. 2024 · Mouse single cells digested from arterial tissues (including ascending aorta, brachiocephalic artery and thoracic aorta) for scRNA-seq were prepared from three ROSA26 ZsGreen1/+; Ldlr-/-; Myh11-CreERT2 mice at the same timepoint of Western diet (WD) … WebSimultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated …
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WebMyh11-creERT2 mice treated with tamoxifen progressively enlarged, whereas none of the vehicle-treated control mice showed any significant variation from their basal condition (Fig-ures 1A–1C). At gross autoptic examination, all aortas of Smad4f/f;Myh11-creERT2 mice treated with tamoxifen displayed one or more
Web(A) Immunofluorescence image of the gastric muscle layer in a Myh11 CreERT2 ;Rosa LSL-YFP/+ mouse prior to tamoxifen injection. Gastric section of a 6-week-old mouse stained for YFP (green) and SMA (red). Nuclei were stained with DAPI. Scale bar = 100 … buck anderson fbiWeb(A) Immunofluorescence image of the gastric muscle layer in a Myh11 CreERT2 ;Rosa LSL-YFP/+ mouse prior to tamoxifen injection. Gastric section of a 6-week-old mouse stained for YFP (green) and SMA (red). Nuclei were stained with DAPI. Scale bar = 100 … extend my wireless home networkWebThis assay will NOT distinguish hemizygous from homozygous transgenic animals. The genotyping protocol (s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends … buck and ewbank 2020Web23 aug. 2024 · Myh11-CreERT2;Tgfbr2 fl/fl transgenic mice were injected with tamoxifen to delete TGF-β receptor ... Laboratory, stock 007576, C57BL background) contain a cell membrane–targeted, 2-color fluorescent Cre-reporter in the ROSA locus. These mice express TdTomato (mT) fluorescent protein in non–Cre recombinase–expressing cells … buck anderson obitWebThe Myh11-CreERT2mouse line (Cre+) has gained increasing application because of its high lineage specificity relative to other Cre drivers targeting smooth muscle cells (SMCs). This Cre allele, however, was initially inserted into the Y chromosome (X/YCre+), which … extend offer letter emailWeblike phenotype on the SAE of injured MYH11-CreERT2:ROSA-TG mice. MYH11-CreERT2:ROSA-TG mice were given 5 daily IP injections of tamoxifen, rested for 5 days, and then injured with naphthalene (300 mg/kg). (A-E) Tracheal sections at 21 days post … extend notice periodWeb29 jul. 2024 · The final genotypes of the SMC lin Tcf21 knockout (SMC lin-KO) mice were: Tg Myh11-CreERT2, Tcf21 ∆SMC/∆SMC, ROSA tdT/+ and ApoE −/ ... extend office 365