Thp1 il-1β
WebApr 14, 2024 · Escartín-Gutiérrez JR, Ponce-Figueroa M, Torres-Vega MÁ, Aguilar-Faisal L, Figueroa-Arredondo P. Transcriptional Activation of a Pro-Inflammatory Response (NF-κB, … WebAug 1, 2011 · Pro-IL-1β is cleaved by the pro-inflammatory protease caspase-1 [3].The activation of caspase-1 occurs via recruitment to a multi-protein complex termed the …
Thp1 il-1β
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WebThe effect of the antioxidant compound, N-acetyl-L-cysteine (NAC), on IL-1beta release and regulation of NF-kappaB in a human myelo-monocytic cell line (THP-1) differentiated into … WebThese results demonstrate that HMGB1 promotes the synthesis of pro-IL-1β and pro-IL-18 in THP-1 macrophages by the activation of p38 MAPK and NF-κB through RAGE. HMGB1 …
WebInvivoGen has developed a cell-based assay to monitor the activation of the NLRC4 inflammasome by intracellular flagellin. This assay relies on two cell lines: • THP1-NLRC4 … WebAug 19, 2016 · Caspase-1 activation and IL-1β production from THP-1 cells by Western blotting and the culture supernatant by enzyme-linked immunosorbent assay analysis …
WebJan 18, 2024 · 不同浓度OMVs作用THP-1细胞时，NLRP3和caspase-1的mRNA表达量较对照组显著升高(P0.05)。作用3 h时，50 μg/mL组NLRP3的mRNA表达量显著高于5 μg/mL … WebMar 12, 2012 · The results showed that the concentrations of TNF-a and IL-1β released from LPS stimulated THP-1 cells increased significantly compared to control (p < 0.01). After …
To further investigate whether RIG-I can affect EV-A71-induced IL-1β release in an NLRP3-independent manner, NLRP3 KD THP-1 macrophages were transfected with siRIG-I and then infected with EV-A71. The expression levels of RIG-I, NLRP3, and EV-A71 VP0 were confirmed by Western blotting (Fig. 3A). The … See more TLRs are known to be implicated in pathogen-induced IL-1β production31. Among these TLRs, evidence suggests that TLR3 and TLR8 play roles in RNA virus … See more TLR engagement has been demonstrated to be sufficient to promote inflammasome activation in human monocytes. To test the effects of TLR3 and TLR8 … See more To determine the potential of viral RNAs in triggering IL-1β release, different amounts of EV-A71 vRNA were transfected into THP-1 macrophages. The expression … See more
Web1.1 材料 人单核细胞(THP-1细胞)购自美国ATCC；DMSO培养基、佛波酯(PMA)购自美国 Sigma公司；FBS、EDTA购自美国Gibcog公司；吡格列酮(Piog，PPAR-γ激动剂) … enchanted florist milton stoke on trentWebApr 30, 2024 · THP-1 cells (5-6 × 10 6 cells/ml) were labeled with 10 μM calcein AM (Sigma-Aldrich, USA) for 45 min at 37° C. After the stimulation, THP-1 cells were added to HAECs … dr brian kellermeyer morgantown wvWebJul 10, 1998 · Perregaux and Gabel (Perregaux, D., and Gabel, C. A. (1994) J. Biol. Chem. 269, 15195–15203) reported that potassium depletion of lipopolysaccharide-stimulated … enchanted florist pentictonWebMar 30, 2024 · THP1 Mφ was cultured with the mixed media for about 8 h for real-time PCR to determine the transcriptional level of Il-1β or 72 h for enzyme linked immunosorbent … enchanted florist las vegasWebPlus particulièrement, les polymorphismes génétiques de l’IL-1β (IL-1β -31 et -511) ne semblent pas influencer l’expression de l’IL-1β de manière significative.Au regard des ... enchanted florist niagara falls nyWebMar 30, 2016 · To ensure the effects of ADAM10 inhibition on ADAM10 cell surface expression and Hla-induced IL-1β was not limited to THP1 cells, we tested whether … dr brian lee pain specialist perthWebSep 27, 2024 · Optimal response to LPS occurs when the CD14 cell surface protein is also present and is characterized by the production of the pro-inflammatory interleukin-8 (IL-8) … dr brian lee fort wayne indiana